Novel Method for Quantifying AhR-Ligand Binding Affinities Using Microscale Thermophoresis

Novel Method for Quantifying AhR-Ligand Binding Affinities Using Microscale Thermophoresis

Blog Article

The aryl hydrocarbon receptor (AhR) is a highly conserved cellular sensor of a variety of environmental pollutants and dietary-, cell- and microbiota-derived metabolites with important roles in fundamental biological processes.Deregulation of the AhR pathway is implicated in several diseases, including autoimmune diseases and cancer, rendering AhR a promising target for drug development and host-directed therapy.The pharmacological intervention of AhR processes requires detailed information NATURAL EYES PIONHOLE GLASSES CHILDRENS about the ligand binding properties to allow specific targeting of a particular signaling process Fenders without affecting the remaining.

Here, we present a novel microscale thermophoresis-based approach to monitoring the binding of purified recombinant human AhR to its natural ligands in a cell-free system.This approach facilitates a precise identification and characterization of unknown AhR ligands and represents a screening strategy for the discovery of potential selective AhR modulators.